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Cripto antibody, Internal

Anti-Cripto antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88209

Cat. No. GTX88209

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 3-5μg/ml

Note :

IHC-P
Human Placenta shows staining assocated with nuclei in some trophoblasts and weak cytoplasm staining in cytotrophoblasts.

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88209-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence YGRNCEHDVRKEN, from the internal region of the protein sequence according to NP_003203.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

teratocarcinoma-derived growth factor 1 , CR , CR-1 , CRGF , CRIPTO

Cellular Localization

Cell membrane , Secreted

Background

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Cripto antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88209

GTX88209 IHC-P Image

IHC-P analysis of human placenta using GTX88209 Cripto antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399