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Cripto antibody

Anti-Cripto antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31511
Anti-Cripto antibody used in Western Blot (WB). GTX31511

Cat No. GTX31511

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit polyclonal Cripto antibody was raised against a 17 amino acid peptide near the amino terminus of human Cripto.The immunogen is located within amino acids 40 - 90 of Cripto.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

teratocarcinoma-derived growth factor 1 , CR , CR-1 , CRGF , CRIPTO

Cellular Localization

Cell membrane,Secreted

Background

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Cripto antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31511

GTX31511 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31511 Cripto antibody.
Working concentration : 20 μg/ml

Anti-Cripto antibody used in Western Blot (WB). GTX31511

GTX31511 WB Image

WB analysis of 293 cell lysate using GTX31511 Cripto antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf