CrkL antibody [5E12]

Cat. No. GTX60462

Host	Mouse
Clonality	Monoclonal
Clone Name	5E12
Isotype	IgG1
Application	WB, FACS, ELISA
Reactivity	Human, Mouse
Package	100 μl ($409)

See all CrkL products

Datasheet
Document

View product citations for antibody GTX60462 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CRKL expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CRK like proto-oncogene, adaptor protein

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]

Database

Gene ID: 1399 CRKL
UniProt: P46109 CRKL

Research Area

DATA IMAGES

GTX60462 WB Image

WB analysis of human CRKL (AA: 100-303) recombinant protein using GTX60462 CrkL antibody [5E12].

GTX60462 ELISA Image

ELISA analysis of antigen using GTX60462 CrkL antibody [5E12].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60462 FACS Image

FACS analysis of NIH3T3 cells using GTX60462 CrkL antibody [5E12].
Blue : CrkL
Red : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty

CrkL antibody [5E12]

Cat. No. GTX60462

Host

Clonality

Clone Name

Isotype

Application

Reactivity