GeneTex

  • Country / Location Selection

United States (US)

CrkL antibody, C-term

Anti-CrkL antibody, C-term used in Western Blot (WB). GTX89690
Anti-CrkL antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89690

Cat. No. GTX89690

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all CrkL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
IHC-P 2.5-3.8μg/ml

Note :

IHC-P
Human Thymus shows staining of cellular membranes in thymocytes.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89690-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KIFDPQNPDENE, from the C Terminus of the protein sequence according to NP_005198.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CRK like proto-oncogene, adaptor protein

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-CrkL antibody, C-term used in Western Blot (WB). GTX89690

GTX89690 WB Image

WB analysis of K562 lysate using GTX89690 CrkL antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-CrkL antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89690

GTX89690 IHC-P Image

IHC-P analysis of human thymus using GTX89690 CrkL antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

REFERENCE

There are currently no references for CrkL antibody, C-term (GTX89690). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CrkL antibody, C-term (GTX89690). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399