CrkL antibody detects CrkL protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CrkL antibody (GTX107677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CrkL antibody detects CrkL protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
CrkL antibody (GTX107677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CrkL antibody detects CrkL protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
CrkL antibody (GTX107677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of CrkL protein from 293T whole cell extracts using 5 μg of CrkL antibody (GTX107677).
Western blot analysis was performed using CrkL antibody (GTX107677).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

CrkL antibody detects CrkL protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CrkL protein stained by CrkL antibody (GTX107677) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody (GTX107677) diluted at 1:1000.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody (GTX107677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the 2020 in Front Mol Biosci. PMID: 33094104