GeneTex
United States (US)

CrkL antibody

GTX107677_42676_20170111_IFA_19011506_600.jpg
GTX107677_39981_IHC_19011506_541.jpg
GTX107677_42676_20170426_IHC-P_19011506_184.jpg
GTX107677_39981_20150407_IP_19011506_843.jpg
GTX107677_42676_20161201_WB_19011506_717.jpg
GTX107677_39981_20151126_WB_M_brain_19011507_583.jpg
GTX107677_39981_IHC_2_19011507_732.jpg

Cat No. GTX107677

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , *U2OS

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377387)

Predict Reactivity

Rat, Zebrafish, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CrkL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Crk Like Proto-Oncogene, Adaptor Protein,Crkl

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Database

Research Area

DATA IMAGES
GTX107677_42676_20170111_IFA_19011506_600.jpg

GTX107677 ICC/IF Image

CrkL antibody detects CrkL protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CrkL protein stained by CrkL antibody (GTX107677) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX107677_39981_IHC_19011506_541.jpg

GTX107677 IHC-P Image

CrkL antibody detects CrkL protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
CrkL antibody (GTX107677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107677_42676_20170426_IHC-P_19011506_184.jpg

GTX107677 IHC-P Image

CrkL antibody detects CrkL protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CrkL antibody (GTX107677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107677_39981_20150407_IP_19011506_843.jpg

GTX107677 IP Image

Immunoprecipitation of CrkL protein from 293T whole cell extracts using 5 μg of CrkL antibody (GTX107677).
Western blot analysis was performed using CrkL antibody (GTX107677).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX107677_42676_20161201_WB_19011506_717.jpg

GTX107677 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody (GTX107677) diluted at 1:1000.

GTX107677_39981_20151126_WB_M_brain_19011507_583.jpg

GTX107677 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody (GTX107677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107677_39981_IHC_2_19011507_732.jpg

GTX107677 IHC-P Image

CrkL antibody detects CrkL protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
CrkL antibody (GTX107677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

CrkL antibody Cat No. GTX107677

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 19-Jan-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : human CrkL transfected HEK293 cells
Amount used : 10μg
Blocking : 2% BSA, 3% non-fat milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane1: HEK293 cells, Lane2: HEK293 cells transfected with pFN21A human CrkL
Date : Anonymous submitted on 17-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf