Untreated (–) and treated (+) K562 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody [HL3176] (GTX640694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.