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Cryptic antibody, Internal

Anti-Cryptic antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89499

Cat. No. GTX89499

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Mouse
Package
100 μg ($399)
See all Cryptic products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2μg/ml

Note :

IHC-P
Mouse Embryonic Liver shows staining of the hepatocyte cell membranes.
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89499-PEP).

Predict Reactivity

Human, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence HFTGRYCEHDQRRSEC, from the internal region of the protein sequence according to NP_115934.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cripto, FRL-1, cryptic family 1 , CFC1B , CRYPTIC , DTGA2 , HTX2

Cellular Localization

Cell membrane , Secreted

Background

This gene encodes a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family, which are involved in signalling during embryonic development. Proteins in this family share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. The protein encoded by this gene is necessary for patterning the left-right embryonic axis. Mutations in this gene are associated with defects in organ development, including autosomal visceral heterotaxy and congenital heart disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-Cryptic antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89499

GTX89499 IHC-P Image

IHC-P analysis of mouse embryo liver using GTX89499 Cryptic antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399