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CtBP1 antibody [N1C1]

Anti-CtBP1 antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX111246
Anti-CtBP1 antibody [N1C1] used in Western Blot (WB). GTX111246
Anti-CtBP1 antibody [N1C1] used in Western Blot (WB). GTX111246
Anti-CtBP1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111246

Cat. No. GTX111246

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all CtBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Raji , mouse brain , rat brain

Predict Reactivity

Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CtBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950053

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-terminal binding protein 1 , BARS , HADDTS

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CtBP1 antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX111246

GTX111246 IHC-Wm Image

Zgc:136929 antibody detects Zgc:136929 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
Zgc:136929 antibody (GTX111246) dilution: 1:200.

Anti-CtBP1 antibody [N1C1] used in Western Blot (WB). GTX111246

GTX111246 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CtBP1 antibody [N1C1] (GTX111246) diluted at 1:1000.

Anti-CtBP1 antibody [N1C1] used in Western Blot (WB). GTX111246

GTX111246 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX111246 diluted at 1:1000

Anti-CtBP1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111246

GTX111246 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CtBP1(GTX111246) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169