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CtBP2 antibody [N1N3]

Anti-CtBP2 antibody [N1N3] used in Western Blot (WB). GTX114346
Anti-CtBP2 antibody [N1N3] used in Western Blot (WB). GTX114346
Anti-CtBP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114346
Anti-CtBP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114346

Cat. No. GTX114346

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HepG2 , HCT116

Product Note

Based on sequence homology, this antibody may cross react with CtBP1 protein.

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CtBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620695

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-terminal binding protein 2

Cellular Localization

Nucleus , Cell junction , synapse

Background

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CtBP2 antibody [N1N3] used in Western Blot (WB). GTX114346

GTX114346 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CtBP2 antibody [N1N3] (GTX114346) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CtBP2 antibody [N1N3] used in Western Blot (WB). GTX114346

GTX114346 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: HCT116
10% SDS PAGE
CTBP2 antibody
GTX114346 diluted at 1:1000

Anti-CtBP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114346

GTX114346 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CtBP2(GTX114346) antibody at 1:500 dilution.

Anti-CtBP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114346

GTX114346 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using CTBP2(GTX114346) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for CtBP2 antibody [N1N3] (GTX114346). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CtBP2 antibody [N1N3] (GTX114346). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169