Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CtBP2 antibody [N1N3] (GTX114346) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using CTBP2(GTX114346) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using CtBP2(GTX114346) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: HCT116
10% SDS PAGE
CTBP2 antibody
GTX114346 diluted at 1:1000