CtIP antibody [19E8]

Cat. No. GTX70264

Reference 7

Host	Mouse
Clonality	Monoclonal
Clone Name	19E8
Isotype	IgG1
Application	WB, ICC/IF, IP, ChIP assay, PLA
Reactivity	Human, Mouse
Package	100 μl ($399)

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View product citations for antibody GTX70264 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:1000
IP	Assay dependent
ChIP assay	Assay dependent
PLA	Assay dependent
Note : WB 3% Milk/TBST overnight

Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human CtIP

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_369822

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB binding protein 8, endonuclease , COM1 , CTIP , JWDS , RIM , SAE2 , SCKL2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5932 RBBP8
UniProt: Q99708 RBBP8

Research Area

DATA IMAGES

GTX70264 ICC/IF Image

CtIP antibody [19E8] detects CtIP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CtIP protein stained by CtIP antibody [19E8] (GTX70264) diluted at 1:100.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX70264 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CtIP antibody [19E8] (GTX70264) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.