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Cullin 3 antibody [GT1229]

Recombinant antibody
Anti-Cullin 3 antibody [GT1229] used in Western Blot (WB). GTX01537
Anti-Cullin 3 antibody [GT1229] used in Western Blot (WB). GTX01537
Anti-Cullin 3 antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX01537
Anti-Cullin 3 antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX01537
Anti-Cullin 3 antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX01537

Cat. No. GTX01537

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1229

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Cullin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Cullin 3.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CUL3,PHA2E,cullin 3

Cellular Localization

Nucleus,Golgi apparatus

Background

This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-Cullin 3 antibody [GT1229] used in Western Blot (WB). GTX01537

GTX01537 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cullin 3 antibody [GT1229] (GTX01537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The double bands were observed. It is possibly due to post translational modification.
Reference: PMID: 29074978 and PMID: 21931660

Anti-Cullin 3 antibody [GT1229] used in Western Blot (WB). GTX01537

GTX01537 WB Image

WB analysis of various samples using GTX01537 Cullin 3 antibody [GT1229].
Dilution : 1:1000
Loading : 25 μg

Anti-Cullin 3 antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX01537

GTX01537 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01537 Cullin 3 antibody [GT1229].
Dilution : 1:100

Anti-Cullin 3 antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX01537

GTX01537 IHC-P Image

IHC-P analysis of human oophoroma tissue section using GTX01537 Cullin 3 antibody [GT1229].
Dilution : 1:100

Anti-Cullin 3 antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX01537

GTX01537 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX01537 Cullin 3 antibody [GT1229].
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399