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Cullin 3 antibody

Anti-Cullin 3 antibody used in Western Blot (WB). GTX103715
Anti-Cullin 3 antibody used in Western Blot (WB). GTX103715

Cat No. GTX103715

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

human CUL3-transfected 293T cells

Predict Reactivity

Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cullin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cullin 3 , CUL-3 , PHA2E

Cellular Localization

Nucleus , Golgi apparatus

Background

CUL3 is a component of a ubiquitin E3 ligase that is essential for mitotic division (Sumara et al., 2007 [PubMed 17543862]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Cullin 3 antibody used in Western Blot (WB). GTX103715

GTX103715 WB Image

Cullin 3 antibody detects Cullin 3 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human CUL3-transfected 293T cells
7.5 % SDS-PAGE
Cullin 3 antibody (GTX103715) dilution: 1:5000

Anti-Cullin 3 antibody used in Western Blot (WB). GTX103715

GTX103715 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cullin 3 antibody (GTX103715) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169