GeneTex
United States (US)

Cullin 3 antibody

GTX103715_39946_WB_B_19011506_848.jpg
GTX103715_39946_20180921_WB_M_testis_19011506_688.jpg

Cat No. GTX103715

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

human CUL3-transfected 293T cells

Predict Reactivity

Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cullin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cullin 3,Cul-3,Pha2E,Cul3

Cellular Localization

Nucleus , Golgi apparatus

Background

CUL3 is a component of a ubiquitin E3 ligase that is essential for mitotic division (Sumara et al., 2007 [PubMed 17543862]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX103715_39946_WB_B_19011506_848.jpg

GTX103715 WB Image

Cullin 3 antibody detects Cullin 3 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human CUL3-transfected 293T cells
7.5 % SDS-PAGE
Cullin 3 antibody (GTX103715) dilution: 1:5000

GTX103715_39946_20180921_WB_M_testis_19011506_688.jpg

GTX103715 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cullin 3 antibody (GTX103715) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169