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Cullin 3 antibody

Anti-Cullin 3 antibody used in Western Blot (WB). GTX53933
Anti-Cullin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53933
Anti-Cullin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX53933
Anti-Cullin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX53933

Cat. No. GTX53933

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Cullin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 569-768 of human CUL3 (NP_003581.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cullin 3 , CUL-3 , PHA2E

Cellular Localization

Nucleus,Golgi apparatus,Cytoplasm

Background

This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-Cullin 3 antibody used in Western Blot (WB). GTX53933

GTX53933 WB Image

WB analysis of various sample lysates using GTX53933 Cullin 3 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Cullin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53933

GTX53933 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX53933 Cullin 3 antibody.
Blue : DAPI

Anti-Cullin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX53933

GTX53933 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX53933 Cullin 3 antibody.
Dilution : 1:100

Anti-Cullin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX53933

GTX53933 IHC-P Image

IHC-P analysis of human prostate tissue using GTX53933 Cullin 3 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399