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Cullin 4a antibody [JU07-33]

Recombinant antibody
Anti-Cullin 4a antibody [JU07-33] used in Western Blot (WB). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in Flow cytometry (FACS). GTX01050
Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

Cat No. GTX01050

Host

Rabbit

Clonality

Monoclonal

Clone Name

JU07-33

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cullin 4A , CUL4A , Cullin 4a

Background

CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-Cullin 4a antibody [JU07-33] used in Western Blot (WB). GTX01050

GTX01050 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cullin 4a antibody [JU07-33] (GTX01050) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The double bands were observed. It is possibly due to post translational modification.
Reference: PMID: 24870930 and PMID: 21487042

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human liver cancer tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human cervix tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX01050 Cullin 4a antibody [JU07-33].

Anti-Cullin 4a antibody [JU07-33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01050

GTX01050 ICC/IF Image

ICC/IF analysis of PFA-fixed HUVEC cells using GTX01050 Cullin 4a antibody [JU07-33].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Cullin 4a antibody [JU07-33] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01050

GTX01050 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX01050 Cullin 4a antibody [JU07-33].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Cullin 4a antibody [JU07-33] used in Flow cytometry (FACS). GTX01050

GTX01050 FACS Image

FACS analysis of HeLa cells using GTX01050 Cullin 4a antibody [JU07-33].
Green : primary antibody
Red : unlabelled control
Dilution : 1:100

Anti-Cullin 4a antibody [JU07-33] used in IHC (Paraffin sections) (IHC-P). GTX01050

GTX01050 IHC-P Image

IHC-P analysis of human breast cancer tissue section using GTX01050 Cullin 4a antibody [JU07-33].

REFERENCE

There are currently no references for Cullin 4a antibody [JU07-33] (GTX01050). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Cullin 4a antibody [JU07-33] (GTX01050). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369