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Cullin 4b antibody

Anti-Cullin 4b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129298
Anti-Cullin 4b antibody used in Western Blot (WB). GTX129298
Anti-Cullin 4b antibody used in Western Blot (WB). GTX129298
Anti-Cullin 4b antibody used in IHC (Paraffin sections) (IHC-P). GTX129298
Anti-Cullin 4b antibody used in Western Blot (WB). GTX129298
Anti-Cullin 4b antibody used in Western Blot (WB). GTX129298

Cat No. GTX129298

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cullin 4b. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cullin 4B , CUL-4B , MRXHF2 , MRXS15 , MRXSC , SFM2

Background

This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cullin 4b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129298

GTX129298 ICC/IF Image

Cullin 4b antibody detects Cullin 4b protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cullin 4b protein stained by Cullin 4b antibody (GTX129298) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Cullin 4b antibody used in Western Blot (WB). GTX129298

GTX129298 WB Image

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
Cullin 4b antibody (GTX129298) dilution: 1:1000

Anti-Cullin 4b antibody used in Western Blot (WB). GTX129298

GTX129298 WB Image

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
Cullin 4b antibody (GTX129298) dilution: 1:1000

Anti-Cullin 4b antibody used in IHC (Paraffin sections) (IHC-P). GTX129298

GTX129298 IHC-P Image

Cullin 4b antibody detects Cullin 4b protein at nucleus on human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma.
Cullin 4b antibody (GTX129298) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cullin 4b antibody used in Western Blot (WB). GTX129298

GTX129298 WB Image

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.
A. 30 μg A375 whole cell lysate/extract
5 % SDS-PAGE
Cullin 4b antibody (GTX129298) dilution: 1:1000

Anti-Cullin 4b antibody used in Western Blot (WB). GTX129298

GTX129298 WB Image

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw 264.7 whole cell lysate/extract
E. 30 μg C2Cl2 whole cell lysate/extract
5 % SDS-PAGE
Cullin 4b antibody (GTX129298) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169