Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cullin 4b antibody (GTX129298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cullin 4b antibody detects Cullin 4b protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cullin 4b protein stained by Cullin 4b antibody (GTX129298) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
Cullin 4b antibody (GTX129298) dilution: 1:1000

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.
A. 30 μg A375 whole cell lysate/extract
5 % SDS-PAGE
Cullin 4b antibody (GTX129298) dilution: 1:1000

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw 264.7 whole cell lysate/extract
E. 30 μg C2Cl2 whole cell lysate/extract
5 % SDS-PAGE
Cullin 4b antibody (GTX129298) dilution: 1:1000

Cullin 4b antibody detects Cullin 4b protein at nucleus on human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma.
Cullin 4b antibody (GTX129298) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min