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Cullin 7 antibody [C2C3], C-term

Anti-Cullin 7 antibody [C2C3], C-term used in Western Blot (WB). GTX113906
Anti-Cullin 7 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113906
Anti-Cullin 7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113906
Anti-Cullin 7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113906

Cat. No. GTX113906

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cullin 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

191 kDa. ( Note )

Positive Control

Molt-4

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Cullin 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036693

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cullin 7 , 3M1 , CUL-7 , KIAA0076 , dJ20C7.5

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of an E3 ubiquitin-protein ligase complex. The encoded protein interacts with TP53, CUL9, and FBXW8 proteins. Defects in this gene are a cause of 3M syndrome type 1 (3M1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cullin 7 antibody [C2C3], C-term used in Western Blot (WB). GTX113906

GTX113906 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX113906 diluted at 1:1000

Anti-Cullin 7 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113906

GTX113906 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cullin 7(GTX113906) antibody at 1:200 dilution.

Anti-Cullin 7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113906

GTX113906 IHC-P Image

Cullin 7 antibody [C2C3], C-term detects Cullin 7 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Cullin 7 antibody [C2C3], C-term (GTX113906) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cullin 7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113906

GTX113906 IHC-P Image

Cullin 7 antibody [C2C3], C-term detects Cullin 7 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Cullin 7 antibody [C2C3], C-term (GTX113906) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zanini IM et al. J Cell Sci 2017; (Epub) Human Cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169