Identification of the Cut5/Rad4 protein in the crude extract of S. pombe with this antibody. Samples were prepared by alkali-lysis of the cells followed by TCA precipitation of proteins. Lane M: Size markers (kDa) Lane 1: Wild-type cells Lane 2: The cut5-5Flag gene replacing the wild-type cut5 gene Lane 3: The cut5-13myc gene replacing the wild type gene Lane 4: The cut-TAP gene replacing the wild-type gene * Cut5 protein is known to be sensitive for protease digestion in the C-terminal region.