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Cut5 / Rad4 (S. pombe) antibody

Anti-Cut5 / Rad4 (S. pombe) antibody used in Western Blot (WB). GTX64104

Cat No. GTX64104

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Saccharomyces pombe
Package
100μl ($349)
APPLICATION

Application Note

Western blotting (500 fold dilution)

Specificity/Sensitivity

Reacts with S. pombe Cut5/Rad4 protein. Not tested for other species
PROPERTIES

Form

Liquid

Buffer

Rabbit antiserum added with 0.05 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Yeast

Immunogen

Recombinant GST-fusion protein with the N-terminal half of Cut5 protein

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Database

DATA IMAGES
Anti-Cut5 / Rad4 (S. pombe) antibody used in Western Blot (WB). GTX64104

GTX64104 WB Image

Identification of the Cut5/Rad4 protein in the crude extract of S. pombe with this antibody. Samples were prepared by alkali-lysis of the cells followed by TCA precipitation of proteins. Lane M: Size markers (kDa) Lane 1: Wild-type cells Lane 2: The cut5-5Flag gene replacing the wild-type cut5 gene Lane 3: The cut5-13myc gene replacing the wild type gene Lane 4: The cut-TAP gene replacing the wild-type gene * Cut5 protein is known to be sensitive for protease digestion in the C-terminal region.

REFERENCE
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REVIEW
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Package List Price ($)
$ 349