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Cyanine antibody (AP)

Anti-Cyanine antibody (AP) used in Western Blot (WB). GTX27242

Cat. No. GTX27242

Host

Sheep

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2500
ELISA 1:2000 - 1:10000
IHC 1:200 - 1:1000
Not tested in other applications.

Product Note

Reactivity was observed against Cy2, Cy3 and Cy5.  Reactivity against other cyanine analogs is unknown.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol pH8.0, 10mg/ml BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A mixture of Cy3 and Cy5 conjugated KLH

Purification

Purified by antigen-affinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Conjugation

Alkaline Phosphatase (AP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Cyanine antibody (AP) used in Western Blot (WB). GTX27242

GTX27242 WB Image

Dot Blot of Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Cy3 Streptavidin Conjugated Row 2 - Cy5 Streptavidin Conjugated. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399