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Cyclin A2 antibody

Orthogonal IP/MS
GTX103042_39981_IP_19011506_733.jpg
GTX103042_40800_20150119_WB_19011506_794.jpg
GTX103042_40800_20161013_WB_Bleomycin_19011506_572.jpg
GTX103042_39981_WB_M_19011506_375.jpg
GTX103042_39981_WB_R_19011506_912.jpg
GTX103042_IHC_19011506_383.jpg
GTX103042_40800_20181221_WB_19011519_886.jpg
GTX103042_39981_20150410_IFA_19011506_851.jpg

Cat No. GTX103042

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , IMR32 , SK-N-AS , HeLa(100 μg/ml Bleomycin treatment for 24 hr) , Neuro2A , PC-12 , *PC3

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377384)

Predict Reactivity

Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin A2,Ccn1,Ccna,Ccna2

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103042_39981_IP_19011506_733.jpg

GTX103042 IP Image

Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103042_40800_20150119_WB_19011506_794.jpg

GTX103042 WB Image

Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103042_40800_20161013_WB_Bleomycin_19011506_572.jpg

GTX103042 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103042_39981_WB_M_19011506_375.jpg

GTX103042 WB Image

Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103042_39981_WB_R_19011506_912.jpg

GTX103042 WB Image

Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103042_IHC_19011506_383.jpg

GTX103042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A (GTX103042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103042_40800_20181221_WB_19011519_886.jpg

GTX103042 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103042_39981_20150410_IFA_19011506_851.jpg

GTX103042 ICC/IF Image

Cyclin A2 antibody detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin A2 protein stained by Cyclin A2 antibody (GTX103042) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

Cyclin A2 antibody Cat No. GTX103042

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 50μg
Blocking : 5% milk, 4°C, overnightHr
Primary Antibody : 1:1500, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169