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Cyclin A2 antibody

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Immunoprecipitation (IP). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103042
Anti-Cyclin A2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

Cat No. GTX103042

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , IMR32 , SK-N-AS , HeLa(100 μg/ml Bleomycin treatment for 24 hr) , Neuro2A , PC-12 , *PC3

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377384)

Predict Reactivity

Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cyclin A2,CCN1,CCNA

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in Immunoprecipitation (IP). GTX103042

GTX103042 IP Image

Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103042

GTX103042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A (GTX103042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclin A2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103042

GTX103042 ICC/IF Image

Cyclin A2 antibody detects Cyclin A2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin A2 stained by Cyclin A2 antibody (GTX103042) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Cyclin A2 antibody Cat No. GTX103042

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 50μg
Blocking : 5% milk, 4°C, overnightHr
Primary Antibody : 1:1500, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169