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Cyclin A2 antibody

Anti-Cyclin A2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103043
Anti-Cyclin A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103043
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103043

Cat No. GTX103043

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Cyclin A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin A2 , CCN1 , CCNA

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin A2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103043

GTX103043 ICC/IF Image

Cyclin A2 antibody detects Cyclin A2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin A2 protein stained by Cyclin A2 antibody (GTX103043) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Cyclin A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103043

GTX103043 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Cyclin A2(GTX103043) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103043

GTX103043 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX103043 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169