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Cyclin B1 antibody [1B10]

Anti-Cyclin B1 antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX82758
Anti-Cyclin B1 antibody [1B10] used in Western Blot (WB). GTX82758

Cat. No. GTX82758

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B10

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Rat
Package
100 μl ($399)
See all Cyclin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CCNB1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin B1 , CCNB

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cyclin B1 antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX82758

GTX82758 IHC-P Image

IHC-P analysis of lung cancer (left) and ovary tumour tissue (right) using GTX82758 Cyclin B1 antibody [1B10].

Anti-Cyclin B1 antibody [1B10] used in Western Blot (WB). GTX82758

GTX82758 WB Image

WB analysis of HeLa (1), Jurkat (2), K562 (3) and PC-12 (4) cell lysate using GTX82758 Cyclin B1 antibody [1B10].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399