GeneTex
United States (US)

Cyclin B1 antibody [CCNB1/1098]

GTX34615_20170712_IHC-P_18121410_442.jpg
GTX34615_20170712_IHC-P_1_18121410_611.jpg
GTX34615_20170712_IHC-P_2_18121410_924.jpg
GTX34615_20170712_IHC-P_3_18121410_785.jpg

Cat No. GTX34615

Host Mouse
Clonality Monoclonal
Clone Name CCNB1/1098
Isotype IgG1
Application IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

IHC-P:0.5-1ug/ml for 30 min at RT.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human full-length CCNB1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin B1,Ccnb,Ccnb1

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34615_20170712_IHC-P_18121410_442.jpg

GTX34615 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)

GTX34615_20170712_IHC-P_1_18121410_611.jpg

GTX34615 IHC-P Image

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)

GTX34615_20170712_IHC-P_2_18121410_924.jpg

GTX34615 IHC-P Image

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)

GTX34615_20170712_IHC-P_3_18121410_785.jpg

GTX34615 IHC-P Image

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)

Package List Price ($)
$ 329