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Cyclin B1 antibody [GT238]

Orthogonal
Anti-Cyclin B1 antibody [GT238] used in IHC (Paraffin sections) (IHC-P). GTX628546
Anti-Cyclin B1 antibody [GT238] used in Western Blot (WB). GTX628546
Anti-Cyclin B1 antibody [GT238] used in Western Blot (WB). GTX628546
Anti-Cyclin B1 antibody [GT238] used in IHC (Paraffin sections) (IHC-P). GTX628546

Cat. No. GTX628546

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT238

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cyclin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa (2mM thymidine treatment and recovery for 3.5hr) , HeLa (2mM thymidine treatment and recovery for 12hr) , U2OS (100ng/ml Nocodazole treatment for 24hr)

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888049

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin B1 , CCNB

Cellular Localization

Cytoplasm , Nucleus , Centrosome

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin B1 antibody [GT238] used in IHC (Paraffin sections) (IHC-P). GTX628546

GTX628546 IHC-P Image

Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin B1 antibody [GT238] used in Western Blot (WB). GTX628546

GTX628546 WB Image

Orthogonal

Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis.
A. 30 μg U2OS whole cell lysate/extract (untreated)
B. 30 μg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
10 % SDS-PAGE
Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:1000

Anti-Cyclin B1 antibody [GT238] used in Western Blot (WB). GTX628546

GTX628546 WB Image

Orthogonal

Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (serum starvation for 48hr)
C. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 3.5hr)
D. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 12hr)
10 % SDS-PAGE
Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:500

Anti-Cyclin B1 antibody [GT238] used in IHC (Paraffin sections) (IHC-P). GTX628546

GTX628546 IHC-P Image

Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ming-YangLee et al. European Journal of Pharmacology 2018; 839 : 57-65 Indirubin-3'-oxime suppresses human cholangiocarcinoma through cell-cycle arrest and apoptosis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations