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Cyclin B1 antibody

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911
Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100911
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

Cat. No. GTX100911

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, African green monkey
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949886

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin B1 , CCNB

Cellular Localization

Cytoplasm , Nucleus , Centrosome

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Sample (50 μg of HeLa whole cell lysate)
GTX100911 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100911

GTX100911 IHC-P Image

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Cyclin B1 antibody (GTX100911) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Cyclin B1 antibody detects Cyclin B1 protein by Western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract |
D. 1 μg 293T whole cell lysate/extract
10 % SDS-PAGE
Cyclin B1 antibody (GTX100911) dilution: 1:3000

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Sample (30 ug of whole cell lysate)
A: U2OS
B: U2OS treated with 100ng/ml Nocodazole for 16hr
10% SDS PAGE
GTX100911 diluted at 1:1000

Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100911

GTX100911 ICC/IF Image

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin B1 stained by Cyclin B1 antibody (GTX100911) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin B1 antibody (GTX100911) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100911

GTX100911 ICC/IF Image

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin B1 stained by Cyclin B1 antibody (GTX100911) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX100911

GTX100911 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin B1 antibody (GTX100911) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Cyclin B1 antibody Cat. No. GTX100911

Rating ( Average 4.7 based on 9 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 9 users reviews)
Date : Anonymous submitted on 24-Oct-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC-3 cells, OECM-1 cells
Amount used : 50μg
Blocking : 5% non-fat milk in TBST, 30°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Date : Anonymous submitted on 07-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description :
Lane 1 : Control
Lane 2 : EGFR (CPT) : ZH2 (CPT) 20:0
Lane 3 : EGFR (CPT) : ZH2 (CPT) 15:5
Lane 4 : EGFR (CPT) : ZH2 (CPT) 10:10
Lane 5 : EGFR (CPT) : ZH2 (CPT) 5:15
Lane 6 : EGFR (CPT) : ZH2 (CPT) 0:20
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal World J Surg Oncol in 2018. PMID: 29466986
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2012. PMID: 22283874
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25105411
Date : Anonymous submitted on 24-Sep-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 12Hr
Date : Anonymous submitted on 17-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-5 cells, CL1-0 cells, A549 cells
Amount used : 10μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 04-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : T84 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169