Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:1000 |
Assay dependent |
1:100 |
1:2000 - 1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
Total lysate protein from HN30 asynchronous cells
Product Note
No reaction was observed against other related cyclins.
Form
Liquid
Buffer
Serum
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
80 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
Purification
Antiserum
This product was prepared from monospecific antiserum by delipidation and defibrination.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
cyclin B1 , CCNB
Cellular Localization
Cytoplasm,Nucleus
Background
In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at aa 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34 cdc2 rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition.
Database
Research Area