GeneTex
United States (US)

Cyclin B1 antibody

GTX27957_18121410_211.jpg

Cat No. GTX27957

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For IHC: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa whole cell lysate

Specificity/Sensitivity

Cyclin B1
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: TLPQLNSALVQDLAKAVAKV, corresponding to C terminal amino acids 414-433 of Human Cyclin B1.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin B1,Ccnb,Ccnb1

Background

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9).

Database

Research Area

DATA IMAGES
GTX27957_18121410_211.jpg

GTX27957 WB Image

WB analysis of HeLa cell lysate using GTX27957 Cyclin B1 antibody.

REFERENCE
Package List Price ($)
$ 319