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Cyclin B1 antibody

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50663
Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50663
Anti-Cyclin B1 antibody used in Western Blot (WB). GTX50663

Cat No. GTX50663

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.145~149 (A-F-S-D-V) derived from human Cyclin B1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin B1 , CCNB

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50663

GTX50663 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50663 Cyclin B1 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Cyclin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50663

GTX50663 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50663 Cyclin B1 antibody.

Anti-Cyclin B1 antibody used in Western Blot (WB). GTX50663

GTX50663 WB Image

WB analysis of extracts from HepG2 and HT-29 cells using GTX50663 Cyclin B1 antibody.

REFERENCE

There are currently no references for Cyclin B1 antibody (GTX50663). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf