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Cyclin B1 antibody

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX73034
Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX73034

Cat No. GTX73034

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human
Package
500 μl ($319)

APPLICATION

Application Note

For IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

48 kDa. ( Note )

Positive Control

Jurkat, HeLa Cells and Breast Carcinoma

PROPERTIES

Form

Liquid

Buffer

10mM Phosphate-buffered saline, pH 7.4, containing 0.2% BSA and <0.1% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide derived from the C-terminus of human cyclin B1

Purification

Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cyclin B1 , Ccnb , Ccnb1

Background

In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is biquitindependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Database

Research Area

DATA IMAGES

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX73034

GTX73034 IHC-P Image

Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin B1 antibody (cat. # GTX73034).

Anti-Cyclin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX73034

GTX73034 IHC-P Image

Human Breast Carcinoma stained with anti-Cyclin B1 antibody (GTX73034)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319