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Cyclin B1 antibody

Cat. No. GTX77612

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Cyclin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/ml
ICC/IF 1:10 - 1:500
IHC-P 1 - 2 μg/ml
FACS 1:10 - 1:1000
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

This is specific to a protein of 62 kD identified as cyclin B1. Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Hamster

Immunogen

Hamster cyclin B1 protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_384564

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin B1 , Ccnb1-rs1 , Ccnb1-rs13 , CycB1 , Cycb-4 , Cycb-5 , Cycb1-rs1

Cellular Localization

Cytoplasm,Nucleus

Background

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Database

Research Area

REFERENCE

Application Reference

Cocchiarella F et al. Molecular Therapy — Methods & Clinical Development 2016; 3 : 16038 Transcriptionally regulated and nontoxic delivery of the hyperactive Sleeping Beauty Transposase.
Application : WB
Reactivity : Human
Benatti P et al. Nucleic Acids Res 2011; 39 (13) : 5356-68 Specific inhibition of NF-Y subunits triggers different cell proliferation defects.
Application : WB
Reactivity : Human
Geley S et al. J Cell Biol 2001; 153 (1) : 137-48 Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
Reactivity : Human

Clone Reference

Brandeis M et al. Proc Natl Acad Sci U S A 1998; 95 (8):892898 Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399