PBS with 100ug BSA 0.1% sodium azide
Keep as concentrated solution, aliquot and store at 4ºC.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at aa 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34 cdc2 rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition.