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Cyclin B1 (phospho Ser147) antibody

Anti-Cyclin B1 (phospho Ser147) antibody used in IHC (Paraffin sections) (IHC-P). GTX55051
Anti-Cyclin B1 (phospho Ser147) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55051
Anti-Cyclin B1 (phospho Ser147) antibody used in Western Blot (WB). GTX55051

Cat. No. GTX55051

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Cyclin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Cyclin B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin B1 , CCNB

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Cyclin B1 (phospho Ser147) antibody used in IHC (Paraffin sections) (IHC-P). GTX55051

GTX55051 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55051 Cyclin B1 (phospho Ser147) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Cyclin B1 (phospho Ser147) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55051

GTX55051 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX55051 Cyclin B1 (phospho Ser147) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Cyclin B1 (phospho Ser147) antibody used in Western Blot (WB). GTX55051

GTX55051 WB Image

WB analysis of anisomycin-treated MDA (A), UV-treated HeLa (B) whole cell lysates using GTX55051 Cyclin B1 (phospho Ser147) antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399