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Cyclin B2 antibody

Anti-Cyclin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111118
Anti-Cyclin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111118
Anti-Cyclin B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111118
Anti-Cyclin B2 antibody used in Western Blot (WB). GTX111118

Cat No. GTX111118

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Bovine, Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949888

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin B2 , HsT17299

Background

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111118

GTX111118 IHC-P Image

Cyclin B2 antibody detects Cyclin B2 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Cyclin B2 antibody (GTX111118) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111118

GTX111118 IHC-P Image

Cyclin B2 antibody detects CCNB2 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Cyclin B2 antibody (GTX111118) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclin B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111118

GTX111118 ICC/IF Image

Cyclin B2 antibody [N1C3-2] detects CCNB2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CCNB2 protein stained by Cyclin B2 antibody [N1C3-2] (GTX111118) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-Cyclin B2 antibody used in Western Blot (WB). GTX111118

GTX111118 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX111118 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169