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Cyclin D1 ELISA Pair

Cyclin D1 ELISA Pair. GTX500039

Cat. No. GTX500039

Application

 ELISA, Sandwich ELISA

Reactivity

 Human
Package
1 pair ($399)
See all Cyclin D1 products
PRODUCT

Product Content

GTX108624
Type : Detection Antibody
Host : Rabbit
Conjugation : Unconjugated
Package : 50 μl
GTX634347
Type : Capture Antibody
Host : Mouse
Conjugation : Unconjugated
Package : 50 μl

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Cyclin D1 , Bcl1 , D11S287E , Prad1 , U21B31 , Ccnd1

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]

Research Area

DATA IMAGES

Cyclin D1 ELISA Pair. GTX500039

GTX500039 ELISA Image

Sandwich ELISA detection of recombinant full-length Cyclin D1 protein using GTX634347 as capture antibody at concentration of 5 μg/mL and GTX108624 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399