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Cyclin D1 antibody [CD1.1]

Cat. No. GTX75314

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CD1.1

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, FACS, IP, ELISA, IHC

Reactivity

 Human, Rat
Package
20 μg ($169)
See all Cyclin D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500
IHC-P 1/1,000-1/1,0000
IHC-Fr Assay dependent
FACS Neat
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377681

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin D1 , BCL1 , D11S287E , PRAD1 , U21B31

Cellular Localization

Nucleus , Cytoplasm , Membrane

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Peterson VM et al. Proc Natl Acad Sci U S A 2013; 110 (51) : E4978-86 Ascites analysis by a microfluidic chip allows tumor-cell profiling.
Wang M et al. Clin Cancer Res 2008; 14 (7) : 2154-60 A severe combined immunodeficient-hu in vivo mouse model of human primary mantle cell lymphoma.
Application : IHC
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 169