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Cyclin D1 antibody [HL2219]

Recombinant antibody KO/KD Comparison
Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223
Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223
Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223
Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223
Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223
Anti-Cyclin D1 antibody [HL2219] used in IHC (Paraffin sections) (IHC-P). GTX638223

Cat. No. GTX638223

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2219

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cyclin D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length Cyclin D1 recombinat protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin D1 , BCL1 , D11S287E , PRAD1 , U21B31

Cellular Localization

Nucleus,Cytoplasm,Membrane

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223

GTX638223 WB Image

KO/KD

Wild-type (WT) and CCND1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [HL2219] (GTX638223) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223

GTX638223 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Cyclin D1 antibody [HL2219] (GTX638223) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223

GTX638223 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [HL2219] (GTX638223) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223

GTX638223 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [HL2219] (GTX638223) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin D1 antibody [HL2219] used in Western Blot (WB). GTX638223

GTX638223 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [HL2219] (GTX638223) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin D1 antibody [HL2219] used in IHC (Paraffin sections) (IHC-P). GTX638223

GTX638223 IHC-P Image

Cyclin D1 antibody [HL2219] detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Cyclin D1 stained by Cyclin D1 antibody [HL2219] (GTX638223) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations