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Cyclin D1 antibody [N1C3]

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Immunoprecipitation (IP). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in ELISA (ELISA). GTX108624

Cat No. GTX108624

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA, sELISA
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX634347 , Detection: GTX108624

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HeLa , MCF-7 , MDA-MB-231 , *Huh 7 , *H184B5F5/M10 , *BT-474 , *HCC1937 , *Hs578T , *MDA-MB-231 , *MDA-MB-361 , *MDA-MB-453 , *T47D , *ZR-75-1

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377392)

Predict Reactivity

Mouse, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cyclin D1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin D1,Bcl1,D11S287E,Prad1,U21B31,Ccnd1

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:1000.

Anti-Cyclin D1 antibody [N1C3] used in Immunoprecipitation (IP). GTX108624

GTX108624 IP Image

Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 μg of Cyclin D1 antibody [N1C3] (GTX108624).
Western blot analysis was performed using Cyclin D1 antibody [N1C3] (GTX108624).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Cyclin D1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108624

GTX108624 ICC/IF Image

Cyclin D1 antibody [N1C3] detects Cyclin D1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

Anti-Cyclin D1 antibody [N1C3] used in ELISA (ELISA). GTX108624

GTX108624 ELISA Image

Sandwich ELISA detection of recombinant full-length Cyclin D1 protein using GTX634347 as capture antibody at concentration of 5 µg/mL and GTX108624 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE
REVIEW

Cyclin D1 antibody [N1C3] Cat No. GTX108624

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample : H184B5F5/M10,BT-474,HCC1937,Hs578T,MCF-7,MDA-MB-231,MDA-MB-361,MDA-MB-453,T47D,ZR-75-1 cells
Amount used : 20μg
Blocking : 1% milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 2Hr
Date : Anonymous submitted on 22-Dec-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169