*Optimal dilutions/concentrations should be determined by the researcher.
Capture: GTX634347 , Detection: GTX108624
Not tested in other applications.
HeLa , MCF-7 , MDA-MB-231 , *Huh 7 , *H184B5F5/M10 , *BT-474 , *HCC1937 , *Hs578T , *MDA-MB-231 , *MDA-MB-361 , *MDA-MB-453 , *T47D , *ZR-75-1
IP/MS validation was supported by references (PMID:30377392)
Mouse, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Full length human Cyclin D1 Recombinant protein.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]