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United States (US)

Cyclin D1 antibody [RM241]

GTX33611_20160517_IHC-P_18121410_790.jpg
GTX33611_20160517_WB_18121410_783.jpg

Cat No. GTX33611

Host Rabbit
Clonality Monoclonal
Clone Name RM241
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

Immunohistochemistry (IHC): 1:500-1:1000 dilution, Western Blot (WB): 1:1000-1:2000 dilution.

Calculated MW

34 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to human Cyclin D1. This antibody may also react to bovine, mouse or rat Cyclin D1, as predicted by immunogen homology.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A peptide corresponding to Cyclin D1

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin D1,Bcl1,D11S287E,Prad1,U21B31,Ccnd1

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX33611_20160517_IHC-P_18121410_790.jpg

GTX33611 IHC-P Image

Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Cyclin D1 RM241 at a 1:1000 dilution.

GTX33611_20160517_WB_18121410_783.jpg

GTX33611 WB Image

Western Blot of HeLa cell lysates using Anti-Cyclin D1 RM241 at a 1:1000 dilution, showed a band of Cyclin D1 (~34 kDa) expressed in HeLa cells.

Package List Price ($)
$ 279