Western blot analysis is shown using GeneTex Anti-Cyclin D1 antibody (GTX21224) to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG GTX27090 and chemiluminescence reagent with a 1min exposure time. Other detection systems will yield similar results.