GeneTex
United States (US)

Cyclin D2 antibody

GTX32545_20180530_IHC-P_1_18121410_107.jpg
GTX32545_20180530_WB_18121410_171.jpg
GTX32545_20180530_IHC-P_18121410_561.jpg

Cat No. GTX32545

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human CCND2 (NP_001750.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin D2,Kiak0002,Mpph3,Ccnd2

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3). [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES
GTX32545_20180530_IHC-P_1_18121410_107.jpg

GTX32545 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32545 Cyclin D2 antibody.
Dilution : 1:100

GTX32545_20180530_WB_18121410_171.jpg

GTX32545 WB Image

WB analysis of various samples using GTX32545 Cyclin D2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32545_20180530_IHC-P_18121410_561.jpg

GTX32545 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32545 Cyclin D2 antibody.
Dilution : 1:100

Package List Price ($)
$ 329