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Cyclin D3 antibody

Anti-Cyclin D3 antibody used in IHC (Paraffin sections) (IHC-P). GTX101522
Anti-Cyclin D3 antibody used in IHC (Paraffin sections) (IHC-P). GTX101522
Anti-Cyclin D3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101522

Cat No. GTX101522

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin D3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cyclin D3

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Cyclin D3 antibody used in IHC (Paraffin sections) (IHC-P). GTX101522

GTX101522 IHC-P Image

Cyclin D3 antibody detects Cyclin D3 protein at nucleus in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
Cyclin D3 antibody (GTX101522) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin D3 antibody used in IHC (Paraffin sections) (IHC-P). GTX101522

GTX101522 IHC-P Image

Cyclin D3 antibody detects Cyclin D3 protein at nucleus in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Cyclin D3 antibody (GTX101522) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin D3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101522

GTX101522 ICC/IF Image

Cyclin D3 antibody detects Cyclin D3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin D3 protein stained by Cyclin D3 antibody (GTX101522) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169