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Cyclin D3 antibody

KO/KD
Anti-Cyclin D3 antibody used in Western Blot (WB). GTX35203
Anti-Cyclin D3 antibody used in Western Blot (WB). GTX35203

Cat. No. GTX35203

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Cyclin D3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human CCND3 (NP_001751.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin D3

Cellular Localization

Nucleus,Cytoplasm,Membrane

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-Cyclin D3 antibody used in Western Blot (WB). GTX35203

GTX35203 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35203 Cyclin D3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Cyclin D3 antibody used in Western Blot (WB). GTX35203

GTX35203 WB Image

WB analysis of various sample lysates using GTX35203 Cyclin D3 antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399