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Cyclin E antibody [HE12]

Orthogonal
Anti-Cyclin E antibody [HE12] used in IHC (Paraffin sections) (IHC-P). GTX23927
Anti-Cyclin E antibody [HE12] used in Western Blot (WB). GTX23927
Anti-Cyclin E antibody [HE12] used in Western Blot (WB). GTX23927
Anti-Cyclin E antibody [HE12] used in Western Blot (WB). GTX23927

Cat. No. GTX23927

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HE12

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
500 μl ($419)
See all Cyclin E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100‐1:200

Note :

IHC-P
Recommendations for antigen retrieval : EDTA buffer pH 8.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to human cyclin E protein.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_385141

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin E1 , CCNE , pCCNE1

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-Cyclin E antibody [HE12] used in IHC (Paraffin sections) (IHC-P). GTX23927

GTX23927 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX23927 Cyclin E antibody [HE12].

Anti-Cyclin E antibody [HE12] used in Western Blot (WB). GTX23927

GTX23927 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28325912

Anti-Cyclin E antibody [HE12] used in Western Blot (WB). GTX23927

GTX23927 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28325912

Anti-Cyclin E antibody [HE12] used in Western Blot (WB). GTX23927

GTX23927 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28325912

REFERENCE

Application Reference

Lee W.J. et al. JournalJournal of Food and Drug Analysis 2021; 29 (1) : 113-127 Tea polyphenol epigallocatechin-3-gallate inhibits cell proliferation in a patient-derived triple-negative breast cancer xenograft mouse model via inhibition of proline-dehydrogenase-induced effects.
Wang CY et al. Drug Des Devel Ther 2020; 14 : 2987-3000 Cordycepin Inhibits Human Gestational Choriocarcinoma Cell Growth by Disrupting Centrosome Homeostasis.
Application : WB
Reactivity : Human
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Hsu CC et al. J Pathol 2019; (Epub) The differential distributions of ASPM isoforms and their roles in Wnt signaling, cell cycle progression, and pancreatic cancer prognosis.
You BJ et al. Sci Rep 2019; 9 (1) : 3306 Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419