GeneTex
United States (US)

Cyclin E antibody

Cat No. GTX27959

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an an assay dependent dilution. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

KNRK, Jurkat, and K-562
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide mapping to the carboxy terminus of rat cyclin E

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CCNE1, CCNE, P24864, CYCLE, 898, 123837, Cyclin E, 25729

Cellular Localization

Nuclear

Background

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Early in the G1 phase of the cell cycle cyclin D1 induction is followed by cyclin E induction (3,4). This sequential progression is marked early on in G1by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB (3,5). The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2 (5,6).
REFERENCE
Package List Price ($)
$ 319