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Cyclin E1 antibody [CCNE1/2460]

Anti-Cyclin E1 antibody [CCNE1/2460] used in Western Blot (WB). GTX18047
Anti-Cyclin E1 antibody [CCNE1/2460] used in IHC (Paraffin sections) (IHC-P). GTX18047
Anti-Cyclin E1 antibody [CCNE1/2460] used in IHC (Paraffin sections) (IHC-P). GTX18047
Anti-Cyclin E1 antibody [CCNE1/2460] used in Protein Array (Protein Array). GTX18047

Cat. No. GTX18047

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CCNE1/2460

Isotype

 IgG2b

Application

 WB, IHC-P, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all Cyclin E1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCNE , CCNE1 , cyclin E1 , pCCNE1 , Cyclin E1

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-Cyclin E1 antibody [CCNE1/2460] used in Western Blot (WB). GTX18047

GTX18047 WB Image

WB analysis of Jurkat cell lysate using GTX18047 Cyclin E1 antibody [CCNE1/2460].

Anti-Cyclin E1 antibody [CCNE1/2460] used in IHC (Paraffin sections) (IHC-P). GTX18047

GTX18047 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX18047 Cyclin E1 antibody [CCNE1/2460].

Anti-Cyclin E1 antibody [CCNE1/2460] used in IHC (Paraffin sections) (IHC-P). GTX18047

GTX18047 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX18047 Cyclin E1 antibody [CCNE1/2460].

Anti-Cyclin E1 antibody [CCNE1/2460] used in Protein Array (Protein Array). GTX18047

GTX18047 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Cyclin E Mouse Monoclonal Antibody (CCNE1/2460). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529