APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide, corresponding to amino acids 90-135 of Human Cyclin E1.
Purification
Purified by antigen-affinity chromatography
From serum
Purity
> 95% (by SDS-PAGE)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cyclin E1 , CCNE , pCCNE1
Cellular Localization
Nucleus
Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]
Database
Research Area
DATA IMAGES
|
GTX66617 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX66617 Cyclin E1 antibody.
Dilution : 1:100
|
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GTX66617 WB Image
WB analysis of various samples using GTX66617 Cyclin E1 antibody.
Lane1 : HEK293T whole cell lysate(20ug)
Lane2 : SK-OVCAR3 whole cell lysate(20ug)
Lane3 : HeLa whole cell lysate(20ug)
Lane4 : PC12 whole cell lysate(20ug)
Lane5 : CT26 whole cell lysate(20ug)
Lane6 : The Testis tissue lysate of Rat(20ug)
Dilution : 1:500
|
REFERENCE
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REVIEW
There are currently no reviews for Cyclin E1 antibody (GTX66617). Be the first to share your experience with this product.
