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Cyclin E1 antibody

Anti-Cyclin E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66617
Anti-Cyclin E1 antibody used in Western Blot (WB). GTX66617

Cat No. GTX66617

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 90-135 of Human Cyclin E1.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin E1 , CCNE , pCCNE1

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-Cyclin E1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66617

GTX66617 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX66617 Cyclin E1 antibody.
Dilution : 1:100

Anti-Cyclin E1 antibody used in Western Blot (WB). GTX66617

GTX66617 WB Image

WB analysis of various samples using GTX66617 Cyclin E1 antibody.
Lane1 : HEK293T whole cell lysate(20ug)
Lane2 : SK-OVCAR3 whole cell lysate(20ug)
Lane3 : HeLa whole cell lysate(20ug)
Lane4 : PC12 whole cell lysate(20ug)
Lane5 : CT26 whole cell lysate(20ug)
Lane6 : The Testis tissue lysate of Rat(20ug)
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349