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Cyclin H antibody [4E11-G2-D7]

Anti-Cyclin H antibody [4E11-G2-D7] used in Western Blot (WB). GTX49246
Anti-Cyclin H antibody [4E11-G2-D7] used in Immunoprecipitation (IP). GTX49246

Cat No. GTX49246

Host

Mouse

Clonality

Monoclonal

Clone Name

4E11-G2-D7

Isotype

IgG2b

Application

WB, IP

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human CCNH(N-term) protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cyclin H , Cak , Cych , P34 , P37 , Ccnh

Cellular Localization

Nucleus.

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[

Database

Research Area

DATA IMAGES

Anti-Cyclin H antibody [4E11-G2-D7] used in Western Blot (WB). GTX49246

GTX49246 WB Image

WB analysis of Jurkat, K562 and A431 lysates using Cyclin H antibody at a dilution of 1:1,000.

Anti-Cyclin H antibody [4E11-G2-D7] used in Immunoprecipitation (IP). GTX49246

GTX49246 IP Image

IP analysis of HeLa lysates using control IgG and Cyclin H antibody. The precipitates were detected by the same Cyclin H antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf