APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2
Predict Reactivity
Mouse, Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.83 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cyclin H. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10722981
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cyclin H , CAK , CycH , p34 , p37
Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Cyclin H antibody [N1C3] used in Western Blot (WB). GTX102754](/templates/default/js/lightbox/images/loading.gif "Anti-Cyclin H antibody [N1C3] used in Western Blot (WB). GTX102754")
|
GTX102754 WB Image
Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX102754 diluted at 1:500
|
REFERENCE
There are currently no references for Cyclin H antibody [N1C3] (GTX102754). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cyclin H antibody [N1C3] (GTX102754). Be the first to share your experience with this product.
![Anti-Cyclin H antibody [N1C3] used in Western Blot (WB). GTX102754](/upload/website/prouct_img/small/GTX102754/GTX102754_40401_WB_19011506_824.jpg "Anti-Cyclin H antibody [N1C3] used in Western Blot (WB). GTX102754")
![Anti-Cyclin H antibody [N1C3] used in Western Blot (WB). GTX102754](/upload/website/prouct_img/normal/GTX102754/GTX102754_40401_WB_19011506_824.jpg "Anti-Cyclin H antibody [N1C3] used in Western Blot (WB). GTX102754")