GeneTex
United States (US)

Cyclin H antibody

GTX53904_20180530_WB_18121410_515.jpg
GTX53904_20180530_IP_18121410_863.jpg

Cat No. GTX53904

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-323 of human CCNH (NP_001230.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P34 Antibody , CAK Antibody , CCNH Antibody , P37 Antibody

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES
GTX53904_20180530_WB_18121410_515.jpg

GTX53904 WB Image

WB analysis of various samples using GTX53904 Cyclin H antibody.
Loading : 25μg per lane

GTX53904_20180530_IP_18121410_863.jpg

GTX53904 IP Image

IP analysis of K562 cell lysate using GTX53904 Cyclin H antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Package List Price ($)
$ 319