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Cyclin H (phospho Thr315) antibody

Anti-Cyclin H (phospho Thr315) antibody used in Western Blot (WB). GTX55052
Anti-Cyclin H (phospho Thr315) antibody used in IHC (Paraffin sections) (IHC-P). GTX55052

Cat No. GTX55052

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate pH7.3, 0.87% Sodium Chloride, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Cyclin H. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin H , CAK , CycH , p34 , p37

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Cyclin H (phospho Thr315) antibody used in Western Blot (WB). GTX55052

GTX55052 WB Image

WB analysis of UV-treated Jurkat (A), UV-treated A549 (B) whole cell lysates using GTX55052 Cyclin H (phospho Thr315) antibody.

Anti-Cyclin H (phospho Thr315) antibody used in IHC (Paraffin sections) (IHC-P). GTX55052

GTX55052 IHC-P Image

IHC-P analysis of formalin fixed human kidney tissue section using GTX55052 Cyclin H (phospho Thr315) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339