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Cyclin L1 (Isoform 1) antibody

Anti-Cyclin L1 (Isoform 1) antibody used in Western Blot (WB). GTX48663
Anti-Cyclin L1 (Isoform 1) antibody used in IHC (Paraffin sections) (IHC-P). GTX48663
Anti-Cyclin L1 (Isoform 1) antibody used in Western Blot (WB). GTX48663

Cat. No. GTX48663

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Drosophila
Package
100 μg ($399)
See all Cyclin L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:5000
IHC-P 20 μg/mL-10 mg/mL
IP 1:100
ELISA 1:5000-1:25000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

No reaction was observed against other related cyclins.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic polypeptide corresponding to an internal region near amino acids 300-375 of Human Cyclin L1a protein.

Purification

Protein A purified
From serum

Conjugation

Unconjugated

RRID

AB_11172711

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin L1 , ANIA6A , BM-001 , PRO1073 , ania-6a

Cellular Localization

Nucleus speckle,nucleoplasm

Background

Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Database

Research Area

DATA IMAGES

Anti-Cyclin L1 (Isoform 1) antibody used in Western Blot (WB). GTX48663

GTX48663 WB Image

WB analysis of mouse brain tissue lysate using GTX48663 Cyclin L1 (Isoform 1) antibody.
Loading : 35 μg
Dilution : 1:3500

Anti-Cyclin L1 (Isoform 1) antibody used in IHC (Paraffin sections) (IHC-P). GTX48663

GTX48663 IHC-P Image

GeneTex's anti-Cyclin L1a antibody was used at a 10 μg/ml to detect Cyclin L1a in a variety of tissues including breast (collagen), heart, kidney (distal tubules), liver, skeletal muscle, ovary μgranulosa and oocyte), pancreas (islet), placenta (trophoblast), prostate (epithelium), skin, spleen (endothelium), stomach (chief), testes (seminiferous epithelium and leydig), thymus (Has-sal's corpuscle and lymphocytes) and uterus μglandular epithelium and stroma). Low to moderate background staining was noted. This image shows Cyclin L1a staining of human spleen. Tissue was formalin-fixed and paraffin embedded.

Anti-Cyclin L1 (Isoform 1) antibody used in Western Blot (WB). GTX48663

GTX48663 WB Image

Western blot using GeneTex's Protein A Purified anti-Cyclin L1a antibody shows detection of a band ~59 kDa corresponding to a Cyclin L1a (arrowhead) present in mouse brain whole cell lysate. Approximately 35μg of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature.

REFERENCE

Application Reference

Vanhoutte D et al. Elife 2016; 5 (Epub) Thrombospondin expression in myofibers stabilizes muscle membranes.
Application : WB
Reactivity : Drosophila
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399