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Cyclin L1 (Isoform 1) antibody

Anti-Cyclin L1 (Isoform 1) antibody used in IHC (Paraffin sections) (IHC-P). GTX48663
Anti-Cyclin L1 (Isoform 1) antibody used in Western Blot (WB). GTX48663

Cat No. GTX48663

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ELISA

Reactivity

Human, Mouse, Drosophila
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2,000 - 1:5,000
IHC-P 10 mg/ml - 20 μg/ml
IP 1:100
ELISA 1:5,000 - 1:25,000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1a protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin L1 , ANIA6A , BM-001 , PRO1073 , ania-6a

Cellular Localization

Nucleus speckle,nucleoplasm

Background

Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Database

DATA IMAGES

Anti-Cyclin L1 (Isoform 1) antibody used in IHC (Paraffin sections) (IHC-P). GTX48663

GTX48663 IHC-P Image

GeneTex's anti-Cyclin L1a antibody was used at a 10 μg/ml to detect Cyclin L1a in a variety of tissues including breast (collagen), heart, kidney (distal tubules), liver, skeletal muscle, ovary μgranulosa and oocyte), pancreas (islet), placenta (trophoblast), prostate (epithelium), skin, spleen (endothelium), stomach (chief), testes (seminiferous epithelium and leydig), thymus (Has-sal's corpuscle and lymphocytes) and uterus μglandular epithelium and stroma). Low to moderate background staining was noted. This image shows Cyclin L1a staining of human spleen. Tissue was formalin-fixed and paraffin embedded.

Anti-Cyclin L1 (Isoform 1) antibody used in Western Blot (WB). GTX48663

GTX48663 WB Image

Western blot using GeneTex's Protein A Purified anti-Cyclin L1a antibody shows detection of a band ~59 kDa corresponding to a Cyclin L1a (arrowhead) present in mouse brain whole cell lysate. Approximately 35μg of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319